Laboratory Medicine ›› 2017, Vol. 32 ›› Issue (1): 22-25.DOI: 10.3969/j.issn.1673-8640.2017.01.006

• Orginal Article • Previous Articles     Next Articles

Establishment and verification of microscopy review rules in AVE automatic urine analysis

LIU Wanchao1, CHEN Longmei2, YANG Zhenhua2, LI Dong1   

  1. 1. Department of Clinical Laboratory,Tongji Hospital,Tongji University,Shanghai 200065,China
    2. Department of Clinical Laboratory,Shanghai Baoshan Traditional Chinese Medicine-Integrated Hospital,Shanghai 201900,China
  • Received:2016-03-31 Online:2017-01-20 Published:2017-02-10

Abstract:

Objective To establish microscopy review rules of AVE automatic urine analysis. Methods Urine dry chemical and urinary sediment analysis was performed for 2 278 urine samples by AVE-752 urine dry chemical analyzer and AVE-766 urinary sediment analyzer. All samples were screened by microscopy with double blind method. AVE-752 and AVE-766 results,modified results and microscopy results were all recorded. Taking the average of microscopy results as standard,the review rules and review rates were established by false negative rates,false positive rates and error rates of 4 schemes(scheme 1:the results of AVE-752 and AVE-766 were analyzed directly;scheme 2:the modified results of AVE-752 and AVE-766 were analyzed;scheme 3:the positive results of scheme 1 were reviewed by microscopy and analyzed; scheme 4:the positive results of scheme 2 were reviewed by microscopy and analyzed),which were based on leucocyte esterase (LEU),occult blood (BLD) and protein (Pro)by AVE-752 and white blood cell(WBC), red blood cell(RBC) and cast(Cast) by AVE-766 and/or modified. A total of 344 urine samples were determined to verify review rules. Results The false positive rates were 20.4%,13.5%,0.0% and 0.0%,the false negative rates were 8.9%,4.2%,8.9% and 4.2%,and the error rates were 16.8%,10.6%,2.8% and 1.3% . The review rates of scheme 3 and scheme 4 were 42.4% and 39.1%,108 samples with false positive results were modified (76 cases of WBC,23 cases of RBC and 9 cases of Cast),and 33 samples with false negative results were modified(8 cases of WBC and 25 cases of RBC). There still existed 30 samples with false negative results (12 cases of WBC and 18 cases of RBC)after modification. The false positive rate of 344 verified urine samples was 0.0%,the false negative rate was 4.5%,the error rate was 1.5%,and the review rate was 35.2%. Conclusions After the combined usage of AVE-752 and AVE-766,all samples should be screened by microscopy except LEU/WBC,BLD/RBC and Pro/Cast.

Key words: Urine routine analysis, Review rule, Review rate

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